DNA

Part:BBa_K1778001:Design

Designed by: Lehua Jia   Group: iGEM15_Scut-Champion-Park   (2015-09-11)


TRE is closely related to promoter regulation,can correctly specific binding to regulatory protein r


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because of the existence of 7 times repeatd sequence,we need to design primers that can be obtained by PCR.


Source

TRE was derived from the commercial plasmid pTRE2, and TRE was the 7 times repeatd TetO sequence.

References